

| Каталожный номер | HB947014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Описание |
Anti-HK1 Polyclonal Antibody (HB947014) is a rabbit polyclonal antibody detecting HK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
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| Реактивность видов | Human, Mouse, Rat | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant Human HK1 (Phe80-Met215). | ||||||||
| Цель | Hexokinase type I, Hexokinase-1, HK1, Brain form hexokinase, Hexokinase-A, HK I | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | P19367 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Фон | Hexokinase-1 (HK1) is a ~102 kDa protein. Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome. 1. Magnani, M. et al. (1992) The Biochemical journal 285 ( Pt 1), 193-9. PMID: 1637300 2. Wang, F. et al. (2014) Investigative ophthalmology & visual science 55, 7159-64. PMID: 25316723 3. Wolf, AJ. et al. (2016) Cell 166, 624-636. PMID: 27374331 | ||||||||
| Примечание | For research use only. |
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