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| カタログ番号 | HC685014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human HLTF (Ala837-Lys996). | ||||||||
| ターゲット | HIP116, RING-type E3 ubiquitin transferase HLTF, SNF2L3, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HLTF, Sucrose nonfermenting protein 2-like 3, ZBU1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Helicase-like transcription factor, RING finger protein 80, SMARCA3, HIP116A, RNF80 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q14527 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF is a ~113 kDa protein. Functions as a DNA-dependent ATPase and E3 ubiquitin-protein ligase involved in chromatin regulation and DNA damage tolerance (DDT). Catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' in response to genotoxic stress, promoting error-free postreplication repair via template switching. Acts as an epigenetic regulator by promoting recruitment of DNMT1, thereby ensuring DNA methylation inheritance: specifically binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance. Catalyzes formation of H3K23ub in two steps: first mediates monoubiquitination together with UBE2E1 and UBE2D2, and then extends ubiquitin chains via 'Lys-63'-linked ubiquitination together with UBE2N and UBE2V2. Also acts as a chromatin redodeling factor, thereby regulating transcription. 1. Unk, I. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3768-73. PMID: 18316726 2. Motegi, A. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12411-6. PMID: 18719106 3. Kile, AC. et al. (2015) Molecular cell 58, 1090-100. PMID: 26051180 4. Hishiki, A. et al. (2020) Journal of biochemistry 167, 597-602. PMID: 31960921 5. Bai, G. et al. (2024) Molecular cell 84, 3044-3060.e11. PMID: 39142279 6. Akano, I. et al. (2025) Molecular cell 85, 2885-2899.e8. PMID: 40680746 7. Ding, H. et al. (1999) The Journal of biological chemistry 274, 19573-80. PMID: 10391891 | ||||||||
| 注意事項 | For research use only. |
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