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Anti-HMBS Polyclonal Antibody (HY082014)

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Aperçu
Numéro de catalogueHY082014
Description
Anti-HMBS Polyclonal Antibody (HY082014) is a rabbit polyclonal antibody detecting HMBS in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human HMBS (Leu85-Ser337).
Cible Hydroxymethylbilane synthase, PBG-D, Pre-uroporphyrinogen synthase, Porphobilinogen deaminase, PBGD, UPS, HMBS
Purification Purified by antigen affinity column.
Numéro d'accès P08397
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Porphobilinogen deaminase (HMBS) is a ~39 kDa protein. As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact.

1. Cunha, L. et al. (2008) Proteins 71, 855-73. PMID: 18004775
2. Song, G. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 396-404. PMID: 18936296
3. Ulbrichova, D. et al. (2009) Blood cells, molecules & diseases 42, 167-73. PMID: 19138865
4. Bustad, HJ. et al. (2013) Bioscience reports 33. PMID: 23815679
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HMBS Polyclonal Antibody

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