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Обзор
Каталожный номерHY082014
Описание
Anti-HMBS Polyclonal Antibody (HY082014) is a rabbit polyclonal antibody detecting HMBS in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human HMBS (Leu85-Ser337).
Цель Hydroxymethylbilane synthase, PBG-D, Pre-uroporphyrinogen synthase, Porphobilinogen deaminase, PBGD, UPS, HMBS
Очистка Purified by antigen affinity column.
Номер доступа P08397
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Porphobilinogen deaminase (HMBS) is a ~39 kDa protein. As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact.

1. Cunha, L. et al. (2008) Proteins 71, 855-73. PMID: 18004775
2. Song, G. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 396-404. PMID: 18936296
3. Ulbrichova, D. et al. (2009) Blood cells, molecules & diseases 42, 167-73. PMID: 19138865
4. Bustad, HJ. et al. (2013) Bioscience reports 33. PMID: 23815679
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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