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Anti-HNF4A Polyclonal Antibody (HW553014)

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Vista general
Número de catálogoHW553014
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HNF4A (Gln131-Ser330).
Objetivo Nuclear receptor subfamily 2 group A member 1, HNF-4-alpha, HNF4A, Hepatocyte nuclear factor 4-alpha, NR2A1, TCF14, Transcription factor 14, Transcription factor HNF-4, TCF-14, HNF4
Purificación Purified by antigen affinity column.
Número de acceso P41235
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Hepatocyte nuclear factor 4-alpha (HNF4A) is a ~52 kDa protein. Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.

1. DeLaForest, A. et al. (2018) Genes 10. PMID: 30597922
2. Qu, M. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E12305-E12312. PMID: 30530698
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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