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Anti-HNRNPF Polyclonal Antibody (HB316014)

Anti-HNRNPF Polyclonal Antibody
Anti-HNRNPF Polyclonal Antibody
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  • 20ug
  • 50ug
  • 100ug
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Vista general
Número de catálogoHB316014
Descripción
Anti-HNRNPF Polyclonal Antibody (HB316014) is a rabbit polyclonal antibody detecting N-terminally processed; HNRNPF; HNRPF in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human HNRNPF (Met1-Asp196).
Objetivo Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed; HNRNPF; HNRPF
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P52597, Q9Z2X1, Q5E9J1, Q794E4
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Heterogeneous nuclear ribonucleoprotein F (HNRNPF) is a ~45 kDa protein. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Nota For research use only
Imágenes
  • Anti-HNRNPF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HNRNPF polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Lncap cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: K562 cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: 4T1 cell lysate
    Lane 6: SH-SY5Y cell lysate
    Lane 7: U2OS cell lysate
    Lane 8: HepG2 cell lysate
    Lane 9: Mouse lung lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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