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Anti-HNRNPF Polyclonal Antibody (HB316014)

Anti-HNRNPF Polyclonal Antibody
Anti-HNRNPF Polyclonal Antibody
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Aperçu
Numéro de catalogueHB316014
Description
Anti-HNRNPF Polyclonal Antibody (HB316014) is a rabbit polyclonal antibody detecting N-terminally processed; HNRNPF; HNRPF in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human HNRNPF (Met1-Asp196).
Cible Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed; HNRNPF; HNRPF
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P52597, Q9Z2X1, Q5E9J1, Q794E4
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Heterogeneous nuclear ribonucleoprotein F (HNRNPF) is a ~45 kDa protein. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Note For research use only
Images
  • Anti-HNRNPF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HNRNPF polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Lncap cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: K562 cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: 4T1 cell lysate
    Lane 6: SH-SY5Y cell lysate
    Lane 7: U2OS cell lysate
    Lane 8: HepG2 cell lysate
    Lane 9: Mouse lung lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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