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Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

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Vista general
Número de catálogoZA251014
Descripción
Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesApis mellifera (Honeybee)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).
Objetivo Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT
Purificación Purified by antigen affinity column.
Número de acceso P01501
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991
2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243
3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482
4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226
5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280
6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135
7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776
8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667
9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Honeybee Api m 4/Melittin Polyclonal Antibody

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