Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

Name: Anti-Honeybee Api m 4/Melittin Polyclonal Antibody
Brand: abinScience
SKU: ZA251014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

ZA251014

Description

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Apis mellifera (Honeybee)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).

Cible

Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT

Purification

Purified by antigen affinity column.

Numéro d'accès

P01501

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991

2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243

3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482

4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226

5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280

6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135

7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776

8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667

9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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