Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

Name: Anti-Honeybee Api m 4/Melittin Polyclonal Antibody
Brand: abinScience
SKU: ZA251014
Price: 168 USD
Availability: InStock

データシート製品共有情報コピー

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

ZA251014

説明

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Apis mellifera (Honeybee)

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).

ターゲット

Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT

精製

Purified by antigen affinity column.

アクセッション番号

P01501

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991

2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243

3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482

4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226

5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280

6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135

7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776

8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667

9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ