Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

Name: Anti-Honeybee Api m 4/Melittin Polyclonal Antibody
Brand: abinScience
SKU: ZA251014
Price: 168 USD
Availability: InStock

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SDS

Обзор

Каталожный номер

ZA251014

Описание

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Apis mellifera (Honeybee)

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).

Цель

Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT

Очистка

Purified by antigen affinity column.

Номер доступа

P01501

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991

2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243

3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482

4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226

5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280

6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135

7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776

8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667

9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа