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Anti-HPV16 Major capsid protein L1 Polyclonal Antibody (VK461014)

Anti-HPV16 Major capsid protein L1 Polyclonal Antibody
Anti-HPV16 Major capsid protein L1 Polyclonal Antibody
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  • 50ug
  • 100ug
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Aperçu
Numéro de catalogueVK461014
Description
Anti-HPV16 L1/Major capsid Polyclonal Antibody L1 Polyclonal Antibody (VK461014) is a rabbit polyclonal antibody detecting Major capsid protein L1 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman papillomavirus 16 (HPV16)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant HPV16 L1/Major capsid protein L1.
Applications testées WB
Cible Major capsid protein L1, L1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P03101
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Major capsid protein L1 is a ~56 kDa protein. Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis.

Note For research use only.
Images
  • Anti-HPV16 Major capsid protein L1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HPC46-L1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HPV16 L1/Major capsid protein L1 (Catalog No: VK461022)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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