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Anti-HSP40/DNAJB1 Polyclonal Antibody (HB704014)

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Vista general
Número de catálogoHB704014
Descripción
Anti-HSP40/DNAJB1 Polyclonal Antibody (HB704014) is a rabbit polyclonal antibody detecting DNAJB1 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HSP40/DNAJB1 (Met1-Ile340).
Objetivo DnaJ protein homolog 1, HSP40, DnaJ homolog subfamily B member 1, DNAJB1, DNAJ1, Heat shock 40 kDa protein 1, hDj-1, HDJ1, Human DnaJ protein 1, Heat shock protein 40, HSPF1
Purificación Purified by antigen affinity column.
Número de acceso P25685
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

DnaJ homolog subfamily B member 1 (HSP40/DNAJB1) is a ~38 kDa protein. Interacts with HSP70 and can stimulate its ATPase activity. Acts also with TTC1 as a chaperone adapter that regulates HSP70-dependent folding process by interacting with the HSP70 amino terminal region. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).

1. Oh, WK. et al. (2003) Molecules and cells 16, 84-91. PMID: 14503850
2. Shi, Y. et al. (1998) Genes & development 12, 654-66. PMID: 9499401
3. Rauch, JN. et al. (2014) The Journal of biological chemistry 289, 1402-14. PMID: 24318877
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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