Anti-HSPA1A Polyclonal Antibody (HY029014)

Name: Anti-HSPA1A Polyclonal Antibody
Brand: abinScience
SKU: HY029014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HY029014

Description

Anti-HSPA1A Polyclonal Antibody (HY029014) is a rabbit polyclonal antibody detecting HSPA1A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Bos mutus grunniens.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human HSPA1A (Met1-Asp641).

Cible

HSP70.1, HSP70-1, HSPA1, HSPA1A, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP72, HSX70

Purification

Purified by antigen affinity column.

Numéro d'accès

P0DMV8

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Heat shock 70 kDa protein 1A (HSPA1A) is a ~70 kDa protein. Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state.

1. Mayer, MP. (2013) Trends in biochemical sciences 38, 507-14. PMID: 24012426

2. Rauch, JN. et al. (2014) The Journal of biological chemistry 289, 1402-14. PMID: 24318877

3. Radons, J. (2016) Cell stress & chaperones 21, 379-404. PMID: 26865365

4. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256

5. Fang, CT. et al. (2016) Cellular and molecular life sciences : CMLS 73, 3949-60. PMID: 27137183

6. Shang, Y. et al. (2014) Biochemical and biophysical research communications 446, 387-92. PMID: 24613385

7. Chen, Z. et al. (2013) Immunity 39, 272-85. PMID: 23973223

8. Shi, Y. et al. (1998) Genes & development 12, 654-66. PMID: 9499401

9. Wang, WF. et al. (2017) Nature communications 8, 363. PMID: 28842558

Note

For research use only.

Images

Reférences

Fiche technique

Anti-HSPA1A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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