Anti-HSPA8 Polyclonal Antibody (HY600014)

Name: Anti-HSPA8 Polyclonal Antibody
Brand: abinScience
SKU: HY600014
Price: 96 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

スペック:

20ug
50ug
100ug

数量:

お問い合わせ

概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY600014

説明

Anti-HSPA8 Polyclonal Antibody (HY600014) is a rabbit polyclonal antibody detecting HSPA8 in ELISA, IHC, WB. Suitable for Bovine, Cricetulus griseus, Danio rerio, Equus caballus, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human HSPA8 (Asp534-Gly615).

ターゲット

Lipopolysaccharide-associated protein 1, HSPA10, Heat shock 70 kDa protein 8, LPS-associated protein 1, LAP-1, HSP73, HSC70, HSPA8, Heat shock cognate 71 kDa protein

精製

Purified by antigen affinity column.

アクセッション番号

P11142

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Heat shock cognate 71 kDa protein (HSPA8) is a ~70 kDa protein. Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated autophagy, activation of proteolysis of misfolded proteins, formation and dissociation of protein complexes, and antigen presentation. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state.

1. Grove, DE. et al. (2011) Molecular biology of the cell 22, 301-14. PMID: 21148293

2. Yamamoto, YH. et al. (2010) Cell structure and function 35, 107-16. PMID: 21150129

3. Sopha, P. et al. (2012) Cell structure and function 37, 177-87. PMID: 23018488

4. Goodwin, EC. et al. (2014) PloS one 9, e94322. PMID: 24732912

5. Li, K. et al. (2017) Molecular cell 65, 52-65. PMID: 27916661

6. Chiang, HL. et al. (1989) Science (New York, N.Y.) 246, 382-5. PMID: 2799391

7. Wang, L. et al. (2023) Molecular cell 83, 281-297.e10. PMID: 36586411

8. Young, JC. et al. (2003) Cell 112, 41-50. PMID: 12526792

10. Rauch, JN. et al. (2014) The Journal of biological chemistry 289, 1402-14. PMID: 24318877

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-HSPA8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 4T1 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Hela cell lysate
Lane 6: U87 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: HepG2 cell lysate
Lane 9: A549 cell lysate
Lane 10: Mouse brain lysate
Lane11: Mouse heart lysate
Lane12: Rat brain lysate
Lane13: Rat heart lysate