Anti-HSPA9/Mortalin/GRP75 Polyclonal Antibody (HW569024)

Name: Anti-HSPA9/Mortalin/GRP75 Polyclonal Antibody
Brand: abinScience
SKU: HW569024
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HW569024

説明

Anti-HSPA9/Mortalin/GRP75 Polyclonal Antibody (HW569024) is a rabbit polyclonal antibody detecting Mortalin in ELISA, IHC, WB. Suitable for Bovine, Dog, Cricetulus griseus, Chicken, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human HSPA9/Mortalin/GRP75 (Lys52-Asp431).

ターゲット

mt-HSP70, HSPA9B, MOT, Peptide-binding protein 74, Stress-70 protein, mitochondrial, PBP74, GRP-75, Mortalin, Heat shock 70 kDa protein 9, GRP75, 75 kDa glucose-regulated protein, HSPA9

精製

Purified by antigen affinity column.

アクセッション番号

P38646

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Stress-70 protein, mitochondrial (HSPA9/Mortalin) is a ~73 kDa protein. Mitochondrial chaperone that plays a key role in mitochondrial protein import, folding, and assembly. Plays an essential role in the protein quality control system, the correct folding of proteins, the re-folding of misfolded proteins, and the targeting of proteins for subsequent degradation. These processes are achieved through cycles of ATP binding, ATP hydrolysis, and ADP release, mediated by co-chaperones. In mitochondria, it associates with the TIM (translocase of the inner membrane) protein complex to assist in the import and folding of mitochondrial proteins. Plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis, interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU.

1. Zhai, P. et al. (2008) The Journal of biological chemistry 283, 26098-106. PMID: 18632665

2. Dores-Silva, PR. et al. (2015) PloS one 10, e0117170. PMID: 25615450

3. Srivastava, S. et al. (2017) The Journal of biological chemistry 292, 18075-18090. PMID: 28848044

4. Moseng, MA. et al. (2019) The journal of physical chemistry. B 123, 3383-3396. PMID: 30933555

5. Moseng, MA. et al. (2019) FEBS letters 593, 2030-2039. PMID: 31177526

6. Shan, Y. et al. (2016) Mitochondrion 26, 94-103. PMID: 26702583

7. Chen, TH. et al. (2011) Blood 117, 1530-9. PMID: 21123823

8. Sane, S. et al. (2014) Cell death & disease 5, e1118. PMID: 24625977

9. Sane, S. et al. (2016) Cell stress & chaperones 21, 313-26. PMID: 26634371

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-Mortalin polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: K562 cell lysate
Lane 3: A549 cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Mouse liver lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse kidney lysate
Lane 9: Rat liver lysate
Lane 10: Rat brain lysate