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Anti-HSPB1/HSP27 Polyclonal Antibody (HY322014)

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Vista general
Número de catálogoHY322014
Descripción
Anti-HSPB1/HSP27 Polyclonal Antibody (HY322014) is a rabbit polyclonal antibody detecting HspB1 in ELISA, IHC, WB. Suitable for Human and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HSPB1/HSP27 (Met1-Lys205).
Objetivo Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, SRP27, Heat shock protein beta-1, Stress-responsive protein 27, HspB1, 28 kDa heat shock protein, HSPB1, HSP27, HSP28, HSP 27
Purificación Purified by antigen affinity column.
Número de acceso P04792
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Heat shock protein beta-1 (HSPB1/HSP27) is a ~22 kDa protein. Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

1. Rogalla, T. et al. (1999) The Journal of biological chemistry 274, 18947-56. PMID: 10383393
2. Almeida-Souza, L. et al. (2010) The Journal of biological chemistry 285, 12778-86. PMID: 20178975
3. Kostenko, S. et al. (2009) Cellular signalling 21, 712-8. PMID: 19166925
4. Holmgren, A. et al. (2013) Acta neuropathologica 126, 93-108. PMID: 23728742
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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