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Anti-HSPB1/HSP27 Polyclonal Antibody (HY322014)

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概要
カタログ番号HY322014
説明
Anti-HSPB1/HSP27 Polyclonal Antibody (HY322014) is a rabbit polyclonal antibody detecting HspB1 in ELISA, IHC, WB. Suitable for Human and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human HSPB1/HSP27 (Met1-Lys205).
ターゲット Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, SRP27, Heat shock protein beta-1, Stress-responsive protein 27, HspB1, 28 kDa heat shock protein, HSPB1, HSP27, HSP28, HSP 27
精製 Purified by antigen affinity column.
アクセッション番号 P04792
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Heat shock protein beta-1 (HSPB1/HSP27) is a ~22 kDa protein. Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

1. Rogalla, T. et al. (1999) The Journal of biological chemistry 274, 18947-56. PMID: 10383393
2. Almeida-Souza, L. et al. (2010) The Journal of biological chemistry 285, 12778-86. PMID: 20178975
3. Kostenko, S. et al. (2009) Cellular signalling 21, 712-8. PMID: 19166925
4. Holmgren, A. et al. (2013) Acta neuropathologica 126, 93-108. PMID: 23728742
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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