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Anti-HSPD1 Polyclonal Antibody (HY166014)

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Aperçu
Numéro de catalogueHY166014
Description
Anti-HSPD1 Polyclonal Antibody (HY166014) is a rabbit polyclonal antibody detecting HSPD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Cricetulus griseus, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human HSPD1 (Ala27-Phe573).
Cible 60 kDa heat shock protein, mitochondrial, HSP-60, HuCHA60, Chaperonin 60, HSP60, Mitochondrial matrix protein P1, Hsp60, HSPD1, 60 kDa chaperonin, CPN60, P60 lymphocyte protein, Heat shock protein 60
Purification Purified by antigen affinity column.
Numéro d'accès P10809
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

60 kDa heat shock protein, mitochondrial (HSPD1) is a ~61 kDa protein. Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10.

1. Levy-Rimler, G. et al. (2001) European journal of biochemistry 268, 3465-72. PMID: 11422376
2. Viitanen, PV. et al. (1992) The Journal of biological chemistry 267, 695-8. PMID: 1346131
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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