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Anti-HSPD1 Polyclonal Antibody (HY166014)

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概要
カタログ番号HY166014
説明
Anti-HSPD1 Polyclonal Antibody (HY166014) is a rabbit polyclonal antibody detecting HSPD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Cricetulus griseus, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human HSPD1 (Ala27-Phe573).
ターゲット 60 kDa heat shock protein, mitochondrial, HSP-60, HuCHA60, Chaperonin 60, HSP60, Mitochondrial matrix protein P1, Hsp60, HSPD1, 60 kDa chaperonin, CPN60, P60 lymphocyte protein, Heat shock protein 60
精製 Purified by antigen affinity column.
アクセッション番号 P10809
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

60 kDa heat shock protein, mitochondrial (HSPD1) is a ~61 kDa protein. Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10.

1. Levy-Rimler, G. et al. (2001) European journal of biochemistry 268, 3465-72. PMID: 11422376
2. Viitanen, PV. et al. (1992) The Journal of biological chemistry 267, 695-8. PMID: 1346131
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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