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Anti-HSV1/HHV-1 ICP0 Polyclonal Antibody (VK083014)

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Vista general
Número de catálogoVK083014
Descripción
Anti-HSV1/HHV-1 ICP0 Polyclonal Antibody (VK083014) is a rabbit polyclonal antibody detecting ICP0 in ELISA, IHC, WB. Suitable for Human herpesvirus 1.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman herpesvirus 1 (HHV-1) (Human herpes simplex virus 1) (HSV-1)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant hsv1/hhv-1 ICP0 (Leu639-Ser762).
Objetivo E3 ubiquitin-protein ligase ICP0, EC:2.3.2.27, Alpha-0 protein, Immediate-early protein IE110, RING-type E3 ubiquitin transferase ICP0, Trans-acting transcriptional protein ICP0, VMW110, ICP0
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P08393
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

E3 ubiquitin-protein ligase ICP0 is a ~78 kDa protein. SUMO-targeted ubiquitin ligase that plays an essential role in nuclear antiviral defense evasion triggered by dsDNA viruses. Acts during the initial stages of lytic infection and the reactivation of latent viral genome. Prevents the antiviral effect of nuclear bodies by degrading host PML, SP100, MORC2 and MORC3. Prevents antiviral response to viral DNA induced by IFI16 by degrading it. Additionally, inhibits host IRF3 nuclear signaling to prevent interferon production by the infected cells.

1. Hembram, DSS. et al. (2020) Journal of molecular biology 432, 1952-1977. PMID: 32001251
2. Sloan, E. et al. (2016) Journal of virology 90, 8621-33. PMID: 27440897
3. Gaidt, MM. et al. (2021) Nature 600, 138-142. PMID: 34759314
4. Roubille, S. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2412258121. PMID: 39589886
Nota For research use only
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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