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Anti-HSV1/HHV-1 ICP0 Polyclonal Antibody (VK083014)

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Aperçu
Numéro de catalogueVK083014
Description
Anti-HSV1/HHV-1 ICP0 Polyclonal Antibody (VK083014) is a rabbit polyclonal antibody detecting ICP0 in ELISA, IHC, WB. Suitable for Human herpesvirus 1.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman herpesvirus 1 (HHV-1) (Human herpes simplex virus 1) (HSV-1)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant hsv1/hhv-1 ICP0 (Leu639-Ser762).
Cible E3 ubiquitin-protein ligase ICP0, EC:2.3.2.27, Alpha-0 protein, Immediate-early protein IE110, RING-type E3 ubiquitin transferase ICP0, Trans-acting transcriptional protein ICP0, VMW110, ICP0
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P08393
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

E3 ubiquitin-protein ligase ICP0 is a ~78 kDa protein. SUMO-targeted ubiquitin ligase that plays an essential role in nuclear antiviral defense evasion triggered by dsDNA viruses. Acts during the initial stages of lytic infection and the reactivation of latent viral genome. Prevents the antiviral effect of nuclear bodies by degrading host PML, SP100, MORC2 and MORC3. Prevents antiviral response to viral DNA induced by IFI16 by degrading it. Additionally, inhibits host IRF3 nuclear signaling to prevent interferon production by the infected cells.

1. Hembram, DSS. et al. (2020) Journal of molecular biology 432, 1952-1977. PMID: 32001251
2. Sloan, E. et al. (2016) Journal of virology 90, 8621-33. PMID: 27440897
3. Gaidt, MM. et al. (2021) Nature 600, 138-142. PMID: 34759314
4. Roubille, S. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2412258121. PMID: 39589886
Note For research use only
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HSV1/HHV-1 ICP0 Polyclonal Antibody

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