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Anti-Mouse HTR1A Polyclonal Antibody (MY581014)

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Vista general
Número de catálogoMY581014
Descripción
Anti-HTR1A Polyclonal Antibody (MY581014) is a rabbit polyclonal antibody detecting 5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; Serotonin receptor 1A; Htr1a; Gpcr18 in ELISA, IHC, WB. Suitable for Mouse and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesMouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant mouse HTR1A (Ile218-Lys345).
Objetivo 5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; Serotonin receptor 1A; Htr1a; Gpcr18
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q64264, P19327
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

5-hydroxytryptamine receptor 1A (HTR1A) is a ~46 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. HTR1A is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission: signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Beta-arrestin family members regulate signaling by mediating both receptor desensitization and resensitization processes.

1. Ase, AR. et al. (2000) Journal of neurochemistry 75, 2415-26. PMID: 11080193
2. Deng, DR. et al. (2007) Neuroscience 147, 388-402. PMID: 17543467
3. Audero, E. et al. (2008) Science (New York, N.Y.) 321, 130-3. PMID: 18599790
4. Richardson-Jones, JW. et al. (2011) The Journal of neuroscience : the official journal of the Society for Neuroscience 31, 6008-18. PMID: 21508226
5. Charest, A. et al. (1993) The Journal of neuroscience : the official journal of the Society for Neuroscience 13, 5164-71. PMID: 8254366
6. Ramboz, S. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14476-81. PMID: 9826725
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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