Anti-Human ABHD2 Polyclonal Antibody (HY050014)

Name: Anti-Human ABHD2 Polyclonal Antibody
Brand: abinScience
SKU: HY050014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY050014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ABHD2 (Ala40-Glu425).

Cible

Monoacylglycerol lipase ABHD2, ABHD2, LABH2, Protein PHPS1-2, 2-arachidonoylglycerol hydrolase, Abhydrolase domain-containing protein 2, Acetylesterase, Lung alpha/beta hydrolase 2, Progesterone-sensitive lipase

Purification

Purified by antigen affinity column.

Numéro d'accès

P08910

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Monoacylglycerol lipase ABHD2 is a ~48 kDa protein. Progesterone-dependent acylglycerol lipase that catalyzes hydrolysis of endocannabinoid arachidonoylglycerol (AG) from cell membrane. Acts as a progesterone receptor: progesterone-binding activates the acylglycerol lipase activity, mediating degradation of 1-arachidonoylglycerol (1AG) and 2-arachidonoylglycerol (2AG) to glycerol and arachidonic acid (AA). Also displays an ester hydrolase activity against acetyl ester, butanoate ester and hexadecanoate ester. Plays a key role in sperm capacitation in response to progesterone by mediating degradation of 2AG, an inhibitor of the sperm calcium channel CatSper, leading to calcium influx via CatSper and sperm activation. May also play a role in smooth muscle cells migration.

1. Miller, MR. et al. (2016) Science (New York, N.Y.) 352, 555-9. PMID: 26989199

2. M, NK. et al. (2016) Bioscience reports 36. PMID: 27247428

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ABHD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Mouse lung lysate
Lane 3: Rat heart lysate

Reférences

Fiche technique

Anti-Human ABHD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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