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Anti-Human ACTL7A Polyclonal Antibody (HP966014)

Anti-Human ACTL7A Polyclonal Antibody
Anti-Human ACTL7A Polyclonal Antibody
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Vista general
Número de catálogoHP966014
Descripción
Anti-Human ACTL7A Polyclonal Antibody (HP966014) is a rabbit polyclonal antibody detecting ACTL7A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ACTL7A (Val72-Phe435).
Objetivo Actin-like-7-alpha, ACTL7A, Actin-like protein 7A
Purificación Purified by antigen affinity column.
Número de acceso Q9Y615
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Actin-like protein 7A (ACTL7A) is a ~48 kDa protein. Essential for normal spermatogenesis and male fertility. Required for normal sperm head morphology, acroplaxome formation, acrosome attachment, and acrosome granule stability. May anchor and stabilize acrosomal adherence to the acroplaxome at least in part by facilitating the presence of F-actin in the subacrosomal space. May play an important role in formation and fusion of Golgi-derived vesicles during acrosome biogenesis.

1. Xin, A. et al. (2020) Science advances 6, eaaz4796. PMID: 32923619
Nota For research use only.
Imágenes
  • Anti-Human ACTL7A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACTL7A polyclonal antibody at 1μg/ml on various samples.
    Lane 1: U87 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: Mouse liver lysate
    Lane 4: Mouse testis lysate
    Lane 5: Rat liver lysate
    Lane 6: Rat testis lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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