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Anti-Human ACTL7A Polyclonal Antibody (HP966014)

Anti-Human ACTL7A Polyclonal Antibody
Anti-Human ACTL7A Polyclonal Antibody
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Aperçu
Numéro de catalogueHP966014
Description
Anti-Human ACTL7A Polyclonal Antibody (HP966014) is a rabbit polyclonal antibody detecting ACTL7A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ACTL7A (Val72-Phe435).
Cible Actin-like-7-alpha, ACTL7A, Actin-like protein 7A
Purification Purified by antigen affinity column.
Numéro d'accès Q9Y615
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Actin-like protein 7A (ACTL7A) is a ~48 kDa protein. Essential for normal spermatogenesis and male fertility. Required for normal sperm head morphology, acroplaxome formation, acrosome attachment, and acrosome granule stability. May anchor and stabilize acrosomal adherence to the acroplaxome at least in part by facilitating the presence of F-actin in the subacrosomal space. May play an important role in formation and fusion of Golgi-derived vesicles during acrosome biogenesis.

1. Xin, A. et al. (2020) Science advances 6, eaaz4796. PMID: 32923619
Note For research use only.
Images
  • Anti-Human ACTL7A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACTL7A polyclonal antibody at 1μg/ml on various samples.
    Lane 1: U87 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: Mouse liver lysate
    Lane 4: Mouse testis lysate
    Lane 5: Rat liver lysate
    Lane 6: Rat testis lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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