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Anti-Human ACTL7A Polyclonal Antibody (HP966014)

Anti-Human ACTL7A Polyclonal Antibody
Anti-Human ACTL7A Polyclonal Antibody
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概要
カタログ番号HP966014
説明
Anti-Human ACTL7A Polyclonal Antibody (HP966014) is a rabbit polyclonal antibody detecting ACTL7A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human ACTL7A (Val72-Phe435).
ターゲット Actin-like-7-alpha, ACTL7A, Actin-like protein 7A
精製 Purified by antigen affinity column.
アクセッション番号 Q9Y615
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Actin-like protein 7A (ACTL7A) is a ~48 kDa protein. Essential for normal spermatogenesis and male fertility. Required for normal sperm head morphology, acroplaxome formation, acrosome attachment, and acrosome granule stability. May anchor and stabilize acrosomal adherence to the acroplaxome at least in part by facilitating the presence of F-actin in the subacrosomal space. May play an important role in formation and fusion of Golgi-derived vesicles during acrosome biogenesis.

1. Xin, A. et al. (2020) Science advances 6, eaaz4796. PMID: 32923619
注意事項 For research use only.
イメージ
  • Anti-Human ACTL7A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACTL7A polyclonal antibody at 1μg/ml on various samples.
    Lane 1: U87 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: Mouse liver lysate
    Lane 4: Mouse testis lysate
    Lane 5: Rat liver lysate
    Lane 6: Rat testis lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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