Anti-Human ACTR3 Polyclonal Antibody (HX211014)

Name: Anti-Human ACTR3 Polyclonal Antibody
Brand: abinScience
SKU: HX211014
Price: 96 USD
Availability: InStock

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20ug
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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HX211014

Descripción

Anti-Human ACTR3 Polyclonal Antibody (HX211014) is a rabbit polyclonal antibody detecting ACTR3 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Xenopus laevis, and Takifugu rubripes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ACTR3 (Pro6-Lys254).

Objetivo

ACTR3

Purificación

Purified by antigen affinity column.

Número de acceso

P61158

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nombres alternativos

Actin-related protein 3, Actin-like protein 3, ACTR3, ARP3

Fondo

Actin-related protein 3 (ACTR3) is a ~47 kDa protein. ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

1. Welch, MD. et al. (1997) Nature 385, 265-9. PMID: 9000076

2. Rao, J. et al. (2017) The Journal of clinical investigation 127, 4257-4269. PMID: 29058690

3. Yoo, Y. et al. (2007) The Journal of biological chemistry 282, 7616-23. PMID: 17220302

4. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

5. Kim, J. et al. (2010) Nature 464, 1048-51. PMID: 20393563

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-ACTR3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: A431 cell lysate
Lane 3: HL60 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K562 cell lysate
Lane 7: PC-3 cell lysate
Lane 8: U2OS cell lysate
Lane 9: Mouse spleen lysate
Lane 10: Mouse lung lysate
Lane11: Mouse liver lysate
Lane12: Rat spleen lysate
Lane13: Rat lung lysate
Lane14: Rat liver lysate

Referencias

Datasheet

Anti-Human ACTR3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo