Anti-Human ACTR3 Polyclonal Antibody (HX211014)

Name: Anti-Human ACTR3 Polyclonal Antibody
Brand: abinScience
SKU: HX211014
Price: 96 USD
Availability: InStock

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Каталожный номер

HX211014

Описание

Anti-Human ACTR3 Polyclonal Antibody (HX211014) is a rabbit polyclonal antibody detecting ACTR3 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Xenopus laevis, and Takifugu rubripes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human ACTR3 (Pro6-Lys254).

Цель

ACTR3

Очистка

Purified by antigen affinity column.

Номер доступа

P61158

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Альтернативные названия

Actin-related protein 3, Actin-like protein 3, ACTR3, ARP3

Фон

Actin-related protein 3 (ACTR3) is a ~47 kDa protein. ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

1. Welch, MD. et al. (1997) Nature 385, 265-9. PMID: 9000076

2. Rao, J. et al. (2017) The Journal of clinical investigation 127, 4257-4269. PMID: 29058690

3. Yoo, Y. et al. (2007) The Journal of biological chemistry 282, 7616-23. PMID: 17220302

4. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

5. Kim, J. et al. (2010) Nature 464, 1048-51. PMID: 20393563

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-ACTR3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: A431 cell lysate
Lane 3: HL60 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K562 cell lysate
Lane 7: PC-3 cell lysate
Lane 8: U2OS cell lysate
Lane 9: Mouse spleen lysate
Lane 10: Mouse lung lysate
Lane11: Mouse liver lysate
Lane12: Rat spleen lysate
Lane13: Rat lung lysate
Lane14: Rat liver lysate

Ссылки

Даташит

Anti-Human ACTR3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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