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| カタログ番号 | HT428323 |
|---|---|
| 種反応性 | Human |
| アプリケーション | FCM |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | VHH-mFc |
| クローンID | SAA1511 |
| 抱合 | PE |
| ターゲット | ADAM 12, MLTN, Meltrin-alpha, Disintegrin and metalloproteinase domain-containing protein 12, ADAM12 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 関連製品 | VHH-mFc Isotype Control antibody (cAb-Lys2), PE |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | O43184 |
| RRID | Anti-Human ADAM12 Antibody (SAA1511), PE (abinScience Cat# HT428323, RRID:AB_3729856) |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 安定性と保存 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 背景 | Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) is a ~99 kDa protein. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. |
| 注意事項 | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), PE (MV080227) |
Flow-cytometry using PE anti-human ADAM12 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAM12 monoclonal antibody (Catalog HT428323, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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