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Anti-Human ADI1 Polyclonal Antibody (HP903014)

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Aperçu
Numéro de catalogueHP903014
Description
Anti-Human ADI1 Polyclonal Antibody (HP903014) is a rabbit polyclonal antibody detecting ADI1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ADI1 (Met1-Ala179).
Cible Sip-L, Acireductone dioxygenase (Fe(2+)-requiring), ADI1, ARD, 1, 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, MTCBP-1, Submergence-induced protein-like factor, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Fe-ARD, MTCBP1
Purification Purified by antigen affinity column.
Numéro d'accès Q9BV57
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Acireductone dioxygenase (ADI1) is a ~21 kDa protein. Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line.

1. Hirano, W. et al. (2005) Genes to cells : devoted to molecular & cellular mechanisms 10, 565-74. PMID: 15938715
2. Uekita, T. et al. (2004) The Journal of biological chemistry 279, 12734-43. PMID: 14718544
3. Yeh, CT. et al. (2001) Journal of virology 75, 11017-24. PMID: 11602742
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ADI1 Polyclonal Antibody

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