Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5) (HY276013)

Name: Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5)
Brand: abinScience
SKU: HY276013
Price: 110 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY276013

Reactividad de especies

Human

Aplicaciones

FCM, ICC, IF, WB

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

AF5

Objetivo

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P02771

RRID

Anti-Human AFP/Alpha-fetoprotein Antibody (AF5) (abinScience Cat# HY276013, RRID:AB_3725843)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Nota

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Imágenes

Bioactivity

SEC-HPLC detection for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).
Flow Cytometry

Flow-cytometry using anti-human AFP antibody.AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog # HY276013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).
Flow CytoMetry

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011)
Lane 2: negative control
Immunofluorescence

Immunofluorescence staining of HepG2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.