Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5) (HY276013)

Name: Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5)
Brand: abinScience
SKU: HY276013
Price: 110 USD
Availability: InStock

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20ug
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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY276013

種反応性

Human

アプリケーション

FCM, ICC, IF, WB

宿主種

Mouse

クローン性

Monoclonal

アイソタイプ

IgG1, kappa

クローンID

AF5

ターゲット

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

P02771

RRID

Anti-Human AFP/Alpha-fetoprotein Antibody (AF5) (abinScience Cat# HY276013, RRID:AB_3725843)

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

注意事項

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

イメージ

Bioactivity

SEC-HPLC detection for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).
Flow Cytometry

Flow-cytometry using anti-human AFP antibody.AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog # HY276013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).
Flow CytoMetry

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011)
Lane 2: negative control
Immunofluorescence

Immunofluorescence staining of HepG2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.