Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5) (HY276013)

Name: Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5)
Brand: abinScience
SKU: HY276013
Price: 110 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HY276013

Реактивность видов

Human

Применения

FCM, ICC, IF, WB

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

AF5

Цель

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P02771

RRID

Anti-Human AFP/Alpha-fetoprotein Antibody (AF5) (abinScience Cat# HY276013, RRID:AB_3725843)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Примечание

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Изображения

Bioactivity

SEC-HPLC detection for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).
Flow Cytometry

Flow-cytometry using anti-human AFP antibody.AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog # HY276013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).
Flow CytoMetry

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011)
Lane 2: negative control
Immunofluorescence

Immunofluorescence staining of HepG2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.