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Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014)

Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody
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Vista general
Número de catálogoHF865014
Descripción
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014) is a rabbit polyclonal antibody detecting AK1/Adenylate kinase isoenzyme 1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human AK1/Adenylate kinase isoenzyme 1 (Met1-Lys194).
Objetivo AK1/Adenylate kinase isoenzyme 1
Purificación Purified by antigen affinity column.
Número de acceso P00568
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosAdenylate kinase isoenzyme 1, AK 1, 2.7.4.3, 2.7.4.6, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase, AK1
Fondo

Adenylate kinase isoenzyme 1 (AK1) is a ~21 kDa protein. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP.

1. Panayiotou, C. et al. (2011) The Biochemical journal 433, 527-34. PMID: 21080915
2. Amiri, M. et al. (2013) The international journal of biochemistry & cell biology 45, 925-31. PMID: 23416111
3. Matsuura, S. et al. (1989) The Journal of biological chemistry 264, 10148-55. PMID: 2542324
Nota For research use only.
Imágenes
  • Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AK1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse skeletal muscle lysate
    Lane 3: Rat heart lysate
    Lane 4: Rat skeletal muscle lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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