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Anti-Human ALDH1A3 Polyclonal Antibody (HW329014)

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Anti-Human ALDH1A3 Polyclonal Antibody
Anti-Human ALDH1A3 Polyclonal Antibody
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Vista general
Número de catálogoHW329014
Descripción
Anti-Human ALDH1A3 Polyclonal Antibody (HW329014) is a rabbit polyclonal antibody detecting ALDH1A3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ALDH1A3 (Lys33-Pro512).
Objetivo Aldehyde dehydrogenase family 1 member A3, Aldehyde dehydrogenase 6, ALDH1A3, RalDH3, ALDH6, Retinaldehyde dehydrogenase 3, RALDH-3
Purificación Purified by antigen affinity column.
Número de acceso P47895
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Retinaldehyde dehydrogenase 3 (ALDH1A3) is a ~56 kDa protein. Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. High specificity for all-trans-retinal as substrate, can also accept acetaldehyde as substrate in vitro but with lower affinity. Required for the biosynthesis of normal levels of retinoate in the embryonic ocular and nasal regions; a critical lipid in the embryonic development of the eye and the nasal region.

1. Moretti, A. et al. (2016) Scientific reports 6, 35710. PMID: 27759097
Nota For research use only.
Imágenes
  • Anti-Human ALDH1A3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ALDH1A3 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: A549 cell lysate
    Lane 2: Mouse liver lysate
    Lane 3: Mouse kidney lysate
    Lane 4: Rat liver lysate
    Lane 5: Rat kidney lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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