中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HD328014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human ALKBH2 Polyclonal Antibody (HD328014) is a rabbit polyclonal antibody detecting ALKBH2. Suitable for Human.
|
||||||||
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ALKBH2 (Ser56-Lys261). | ||||||||
| ターゲット | ABH2, ALKBH2, Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, DNA oxidative demethylase ALKBH2, EC:1.14.11.33, Oxy DC1 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q6NS38 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 背景 | DNA oxidative demethylase ALKBH2 is a ~29 kDa protein. Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. 1. Duncan, T. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 16660-5. PMID: 12486230 2. Aas, PA. et al. (2003) Nature 421, 859-63. PMID: 12594517 3. Lee, DH. et al. (2005) The Journal of biological chemistry 280, 39448-59. PMID: 16174769 4. Chen, B. et al. (2010) Molecular bioSystems 6, 2143-9. PMID: 20714506 5. Li, P. et al. (2013) Cell reports 4, 817-29. PMID: 23972994 6. Zdżalik, D. et al. (2015) DNA repair 30, 1-10. PMID: 25797601 7. Yang, CG. et al. (2008) Nature 452, 961-5. PMID: 18432238 8. Yi, C. et al. (2012) Nature structural & molecular biology 19, 671-6. PMID: 22659876 | ||||||||
| 注意事項 | For research use only |
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
