

| Каталожный номер | HD328014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Описание |
Anti-Human ALKBH2 Polyclonal Antibody (HD328014) is a rabbit polyclonal antibody detecting ALKBH2. Suitable for Human.
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| Реактивность видов | Human | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant Human ALKBH2 (Ser56-Lys261). | ||||||||
| Цель | ABH2, ALKBH2, Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, DNA oxidative demethylase ALKBH2, EC:1.14.11.33, Oxy DC1 | ||||||||
| Уровень эндотоксинов | Please contact with the lab for this information. | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | Q6NS38 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Фон | DNA oxidative demethylase ALKBH2 is a ~29 kDa protein. Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. 1. Duncan, T. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 16660-5. PMID: 12486230 2. Aas, PA. et al. (2003) Nature 421, 859-63. PMID: 12594517 3. Lee, DH. et al. (2005) The Journal of biological chemistry 280, 39448-59. PMID: 16174769 4. Chen, B. et al. (2010) Molecular bioSystems 6, 2143-9. PMID: 20714506 5. Li, P. et al. (2013) Cell reports 4, 817-29. PMID: 23972994 6. Zdżalik, D. et al. (2015) DNA repair 30, 1-10. PMID: 25797601 7. Yang, CG. et al. (2008) Nature 452, 961-5. PMID: 18432238 8. Yi, C. et al. (2012) Nature structural & molecular biology 19, 671-6. PMID: 22659876 | ||||||||
| Примечание | For research use only |
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