Anti-Human ALKBH5 Polyclonal Antibody (HA341014)

Name: Anti-Human ALKBH5 Polyclonal Antibody
Brand: abinScience
SKU: HA341014
Price: 168 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HA341014

Description

Anti-Human ALKBH5 Polyclonal Antibody (HA341014) is a rabbit polyclonal antibody detecting ALKBH5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ALKBH5 (Met1-His394).

Cible

ALKBH5, ABH5, OFOXD1, RNA demethylase ALKBH5, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5

Purification

Purified by antigen affinity column.

Numéro d'accès

Q6P6C2

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

RNA demethylase ALKBH5 is a ~44 kDa protein. Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing, translation and export. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.

1. Zheng, G. et al. (2013) Molecular cell 49, 18-29. PMID: 23177736

2. Aik, W. et al. (2014) Nucleic acids research 42, 4741-54. PMID: 24489119

3. Feng, C. et al. (2014) The Journal of biological chemistry 289, 11571-11583. PMID: 24616105

4. Xu, C. et al. (2014) The Journal of biological chemistry 289, 17299-311. PMID: 24778178

5. Yu, F. et al. (2021) Nucleic acids research 49, 5779-5797. PMID: 34048572

6. Cieśla, M. et al. (2023) Molecular cell 83, 1165-1179.e11. PMID: 36944332

7. Yu, F. et al. (2023) Molecular cell 83, 2003-2019.e6. PMID: 37257451

8. Zhang, XL. et al. (2023) Nature communications 14, 3815. PMID: 37369679

9. Sun, H-Y. et al. (2025) Journal of virology 99, e0050725. PMID: 40793791

10. Thalhammer, A. et al. (2011) PloS one 6, e16210. PMID: 21264265

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human ALKBH5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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