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| カタログ番号 | HP629014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human AMFR Polyclonal Antibody (HP629014) is a rabbit polyclonal antibody detecting AMFR in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human AMFR (Gln456-Ser643). | ||||||||
| ターゲット | AMFR | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9UKV5 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 別名 | E3 ubiquitin-protein ligase AMFR, 2.3.2.36, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45 | ||||||||
| 背景 | E3 ubiquitin-protein ligase AMFR is a ~72 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane. In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. 1. Shimizu, K. et al. (1999) FEBS letters 456, 295-300. PMID: 10456327 2. Fang, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 14422-7. PMID: 11724934 3. Liang, JS. et al. (2003) The Journal of biological chemistry 278, 23984-8. PMID: 12670940 4. Pabarcus, MK. et al. (2009) Archives of biochemistry and biophysics 483, 66-74. PMID: 19103148 5. Liu, Y. et al. (2014) eLife 3, e01369. PMID: 24424410 6. Wang, YJ. et al. (2017) Nature cell biology 19, 808-819. PMID: 28604676 7. Song, BL. et al. (2005) Molecular cell 19, 829-40. PMID: 16168377 9. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-AMFR polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
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