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Anti-Human AP2A1 Polyclonal Antibody (HF867014)

Anti-Human AP2A1 Polyclonal Antibody
Anti-Human AP2A1 Polyclonal Antibody
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Vista general
Número de catálogoHF867014
Descripción
Anti-Human AP2A1 Polyclonal Antibody (HF867014) is a rabbit polyclonal antibody detecting AP2A1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human AP2A1 (Ile737-Phe977).
Objetivo AP-2 complex subunit alpha-1, Alpha1-adaptin, Adaptor protein complex AP-2 subunit alpha-1, 100 kDa coated vesicle protein A, Clathrin assembly protein complex 2 alpha-A large chain, ADTAA, CLAPA1, AP2A1, Adaptor-related protein complex 2 subunit alpha-1, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit, Alpha-adaptin A
Purificación Purified by antigen affinity column.
Número de acceso O95782
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

AP-2 complex subunit alpha-1 (AP2A1) is a ~107 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.

1. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497
Nota For research use only.
Imágenes
  • Anti-Human AP2A1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AP2A1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: SKOV3 cell lysate
    Lane 2: THP-1 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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