Anti-Human AP2A1 Polyclonal Antibody (HF867014)

Name: Anti-Human AP2A1 Polyclonal Antibody
Brand: abinScience
SKU: HF867014
Price: 96 USD
Availability: InStock

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Каталожный номер

HF867014

Описание

Anti-Human AP2A1 Polyclonal Antibody (HF867014) is a rabbit polyclonal antibody detecting AP2A1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human AP2A1 (Ile737-Phe977).

Цель

AP-2 complex subunit alpha-1, Alpha1-adaptin, Adaptor protein complex AP-2 subunit alpha-1, 100 kDa coated vesicle protein A, Clathrin assembly protein complex 2 alpha-A large chain, ADTAA, CLAPA1, AP2A1, Adaptor-related protein complex 2 subunit alpha-1, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit, Alpha-adaptin A

Очистка

Purified by antigen affinity column.

Номер доступа

O95782

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

AP-2 complex subunit alpha-1 (AP2A1) is a ~107 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.

1. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-AP2A1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: SKOV3 cell lysate
Lane 2: THP-1 cell lysate

Ссылки

Даташит

Anti-Human AP2A1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Human AP2A1 Polyclonal Antibody (HF867014)

Обзор

Изображения

Ссылки

Даташит

SDS