Anti-Human ApoE3 Recombinant Antibody (1D7) (HF739033)

Name: Anti-Human ApoE3 Recombinant Antibody (1D7)
Brand: abinScience
SKU: HF739033
Price: 110 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

20ug
50ug
100ug
1mg

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HF739033

Reactivité des espèces

Human

Applications

Blocking, ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

1D7

Cible

Apo-E, Apolipoprotein E, APOE

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P02649

RRID

Anti-Human APOE Antibody (1D7) (abinScience Cat# HF739033, RRID:AB_3729106)
_x005F_x005F_x000D_

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Apolipoprotein E (APOE) is a ~36 kDa protein. APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.

1. Krimbou, L. et al. (2004) Journal of lipid research 45, 839-48. PMID: 14754908

2. Arnon, R. et al. (1991) Biochimica et biophysica acta 1085, 336-42. PMID: 1911868

3. Marcel, YL. et al. (1983) Biochimica et biophysica acta 750, 411-7. PMID: 6860692

4. Sehayek, E. et al. (1991) The Journal of biological chemistry 266, 18259-67. PMID: 1917954

5. Verghese, PB. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1807-16. PMID: 23620513

6. Kowal, RC. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5810-4. PMID: 2762297

7. Ji, ZS. et al. (1997) The Journal of biological chemistry 272, 31285-92. PMID: 9395455

8. Li, X. et al. (2003) Biochemistry 42, 10406-17. PMID: 12950167

Note

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Images

SDS-PAGE

SDS-PAGE for Anti-Human APOE Antibody (1D7)
Western blot

Western blot analysis was performed using anti-APOE4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human APOE4 (Catalog No: HF739022)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human APOE antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human APOE monoclonal antibody (Catalog HF739033, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.