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Anti-Human AQP8 Polyclonal Antibody (HC039014)

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Vista general
Número de catálogoHC039014
Descripción
Anti-Human AQP8 Polyclonal Antibody (HC039014) is a rabbit polyclonal antibody detecting Aquaporin-8; AQP-8; AQP8 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human AQP8 (Glu58-Gly84&Lys129-Gly156&Ser205-Trp228).
Objetivo Aquaporin-8; AQP-8; AQP8
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O94778
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Aquaporin-8 (AQP8) is a ~27 kDa protein. Channel that allows the facilitated permeation of water and uncharged molecules, such as hydrogen peroxide and the neutral form of ammonia (NH3), through cellular membranes such as plasma membrane, inner mitochondrial membrane and endoplasmic reticulum membrane of several tissues. The transport of the ammonia neutral form induces a parallel transport of proton, at alkaline pH when the concentration of ammonia is high. However, it is unclear whether the transport of proton takes place via the aquaporin or via an endogenous pathway. Also, may transport ammonia analogs such as formamide and methylamine, a transport favourited at basic pH due to the increase of unprotonated (neutral) form, which is expected to favor diffusion. Does not transport urea or glycerol.

1. Liu, K. et al. (2006) Biology of the cell 98, 153-61. PMID: 15948717
2. Larocca, MC. et al. (2009) American journal of physiology. Gastrointestinal and liver physiology 296, G93-100. PMID: 18948439
3. Bertolotti, M. et al. (2013) Antioxidants & redox signaling 19, 1447-51. PMID: 23541115
4. Medraño-Fernandez, I. et al. (2016) Antioxidants & redox signaling 24, 1031-44. PMID: 26972385
5. Bestetti, S. et al. (2018) Science advances 4, eaar5770. PMID: 29732408
6. Danielli, M. et al. (2019) Free radical biology & medicine 131, 370-375. PMID: 30579780
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human AQP8 Polyclonal Antibody

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