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| カタログ番号 | HP478014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human ATG4A Polyclonal Antibody (HP478014) is a rabbit polyclonal antibody detecting ATG4A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Pongo abelii.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ATG4A (Met1-Val398). | ||||||||
| ターゲット | hAPG4A, Cysteine protease ATG4A, ATG4A, AUTL2, Autophagin-2, APG4A, AUT-like 2 cysteine endopeptidase, Autophagy-related protein 4 homolog A, Autophagy-related cysteine endopeptidase 2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q8WYN0 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Cysteine protease ATG4A is a ~45 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. 1. Scherz-Shouval, R. et al. (2003) The Journal of biological chemistry 278, 14053-8. PMID: 12473658 2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837 3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651 4. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865 5. Shu, CW. et al. (2011) Journal of biomolecular screening 16, 174-82. PMID: 21245471 6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004 7. Nguyen, N. et al. (2020) The Journal of biological chemistry 295, 13584-13600. PMID: 32732290 9. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106 10. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288 | ||||||||
| 注意事項 | For research use only. |
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