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Anti-Human ATG4D Polyclonal Antibody (HC229014)

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Aperçu
Numéro de catalogueHC229014
Description
Anti-Human ATG4D Polyclonal Antibody (HC229014) is a rabbit polyclonal antibody detecting ATG4D. Suitable for Human.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ATG4D (Ser92-Ser420).
Cible APG4D, ATG4D, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease ATG4D, mitochondrial, EC:3.4.22.-, HsAPG4D
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q86TL0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Cysteine protease ATG4D is a ~52 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy.

1. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865
2. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288
3. Agrotis, A. et al. (2019) Autophagy 15, 976-997. PMID: 30661429
4. Durgan, J. et al. (2021) Molecular cell 81, 2031-2040.e8. PMID: 33909989
5. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106
Note For research use only
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