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Anti-Human ATP1B1 Polyclonal Antibody (HY156014)

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Anti-Human ATP1B1 Polyclonal Antibody
Anti-Human ATP1B1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHY156014
Description
Anti-Human ATP1B1 Polyclonal Antibody (HY156014) is a rabbit polyclonal antibody detecting ATP1B1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ATP1B1 (Gly76-Ser303).
Cible Sodium/potassium-transporting ATPase subunit beta-1, ATP1B1, Sodium/potassium-dependent ATPase subunit beta-1, ATP1B
Purification Purified by antigen affinity column.
Numéro d'accès P05026
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Sodium/potassium-transporting ATPase subunit beta-1 (ATP1B1) is a ~35 kDa protein. This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1.

1. Bab-Dinitz, E. et al. (2009) Biochemistry 48, 8684-91. PMID: 19694409
2. Cao, W. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 206, 2668-2681. PMID: 34011520
Note For research use only.
Images
  • Anti-Human ATP1B1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ATP1B1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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