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Anti-Human ATP5F1A Polyclonal Antibody (HD604014)

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Anti-Human ATP5F1A Polyclonal Antibody
Anti-Human ATP5F1A Polyclonal Antibody
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoHD604014
Descripción
Anti-Human ATP5F1A Polyclonal Antibody (HD604014) is a rabbit polyclonal antibody detecting ATP5F1A. Suitable for Rat, Human, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ATP5F1A (Gln44-Ala553).
Objetivo ATP synthase F(1) complex subunit alpha, mitochondrial, ATP synthase F1 subunit alpha, ATP5A, ATP5A1, ATP5AL2, ATP5F1A, ATPM
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P25705
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

ATP synthase F(1) complex subunit alpha, mitochondrial (ATP5F1A) is a ~59 kDa protein. Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
2. Qi, B. et al. (2018) Cell 175, 571-582.e11. PMID: 30146159
Nota For research use only
Imágenes
  • Anti-Human ATP5F1A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ATP5F1A polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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