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Anti-Human ATP5F1A Polyclonal Antibody (HD604014)

Anti-Human ATP5F1A Polyclonal Antibody
Anti-Human ATP5F1A Polyclonal Antibody
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  • 100ug
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Aperçu
Numéro de catalogueHD604014
Description
Anti-Human ATP5F1A Polyclonal Antibody (HD604014) is a rabbit polyclonal antibody detecting ATP5F1A. Suitable for Rat, Human, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ATP5F1A (Gln44-Ala553).
Cible ATP synthase F(1) complex subunit alpha, mitochondrial, ATP synthase F1 subunit alpha, ATP5A, ATP5A1, ATP5AL2, ATP5F1A, ATPM
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P25705
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

ATP synthase F(1) complex subunit alpha, mitochondrial (ATP5F1A) is a ~59 kDa protein. Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
2. Qi, B. et al. (2018) Cell 175, 571-582.e11. PMID: 30146159
Note For research use only
Images
  • Anti-Human ATP5F1A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ATP5F1A polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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