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Anti-Human ATP5F1A Polyclonal Antibody
Anti-Human ATP5F1A Polyclonal Antibody
Цена(USD): --
Спецификация:
  • 50ug
  • 100ug
Количество:
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  • Даташит

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Обзор
Каталожный номерHD604014
Описание
Anti-Human ATP5F1A Polyclonal Antibody (HD604014) is a rabbit polyclonal antibody detecting ATP5F1A. Suitable for Rat, Human, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human ATP5F1A (Gln44-Ala553).
Цель ATP synthase F(1) complex subunit alpha, mitochondrial, ATP synthase F1 subunit alpha, ATP5A, ATP5A1, ATP5AL2, ATP5F1A, ATPM
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P25705
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

ATP synthase F(1) complex subunit alpha, mitochondrial (ATP5F1A) is a ~59 kDa protein. Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
2. Qi, B. et al. (2018) Cell 175, 571-582.e11. PMID: 30146159
Примечание For research use only
Изображения
  • Anti-Human ATP5F1A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ATP5F1A polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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